General Introduction: Protocols for Antibody Production

Antibodies are serum immunoglobulins that have binding specificity for specific antigens. Therefore, antibodies are very useful in applications such as experimental medicine, biology, biomedical research, therapy, and diagnostic testing.

Polyclonal antibodies and monoclonal antibodies can be used for this purpose, although the production of these antibodies requires the use of large numbers of animals with significant welfare implications. You can also visit to get the best service of antibody production.

Antibody Production. - ppt download

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In the case of PAbs, animals are injected with an antigen or an antigen/adjuvant mixture to induce a strong antibody response and it is usually essential to collect blood to monitor the antibody response during the experiment.

In the case of MAbs, animals were injected with an antigen or an antigen/adjuvant mixture to induce specific B cells derived from the spleen or lymph nodes to detect hybridomas.

If in vitro production is not possible, then it is necessary to use animals for the production of antibodies by hybridomas in the abdominal cavity.

When choosing between the production of PAbs or MAbs, the desired antibody application, as well as the time and cost required for production, must be taken into account.

The fact that polyclonal antiserum can be found in a short time with little financial expenditure supports its use, whereas the creation of MAbs takes about 3 to 6 months. Many research questions can be answered with the help of polyclonal antiserum.

MAbs are epitope-specific, which can be important in certain cases. For additional information, we refer readers to detailed comparisons of PAbs and MAbs elsewhere in this issue of the ILAR Journal.

In the production of polyclonal and monoclonal antibodies, a number of important steps can be identified that can affect the results of animal experiments (immunological outcomes and animal pain and suffering).

Conclusion: It evaluates the critical steps in the production of these antibodies and provides recommendations for optimizing the production protocol which ultimately leads to effective antibody response and minimal pain and suffering for the animals.